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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Dev Biol. 2013 Nov 21;386(1):165–180. doi: 10.1016/j.ydbio.2013.11.006

Fig. 9. Addition of PA simulates PLC activity and increases PLCγ in DRM.

Fig. 9

(A) Addition of dPA (400 μM; closed circle) or sperm (closed triangle) increased IP3 mass to equivalent levels when compared to a control group of Xenopus eggs (time zero) (asterisks denote P< 0.002). However, dPS (600 μM; closed square) did not alter IP3 mass. (B) Xenopus eggs (time zero) or after addition of dPA (400 μM), DRM were isolated by density gradient centrifugation and Western blotting with densitometry recorded total Src or PLCγ. Experimental values (n=3–4 per group) were relative to the amount in eggs and asterisks denote significance (P< 0.02).