Skip to main content
. Author manuscript; available in PMC: 2014 Aug 16.
Published in final edited form as: ACS Chem Biol. 2013 May 29;8(8):1730–1736. doi: 10.1021/cb4000986

Figure 2. Alverine and Benfluorex activated the human insulin promoter.

Figure 2

A) Alverine and benfluorex activated the insulin promoter-GFP transgene. To confirm that alverine and benfluorex activated the insulin promoter-GFP transgene, they were added to T6PNE cells at 10 and 20µM for 3 days in the presence of 0.5µM tamoxifen. Activation was dose-dependent as seen by the number of GFP-positive cells (green GFP and corresponding bright field (BF) images). Scale bars=200µm. B) Alverine and benfluorex activated the endogenous human insulin promoter. Drugs were added to T6PNE cells (20µM for 2 days) followed by harvest of RNA for quantitative RT-PCR. Values represent the mean ± SEM, *p<0.05, n=3 biological replicates.