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. Author manuscript; available in PMC: 2014 Feb 12.
Published in final edited form as: Circulation. 2003 Apr 28;107(17):2181–2184. doi: 10.1161/01.CIR.0000070591.21548.69

Figure 2.

Figure 2

Phenotype of the calcified aortic valve. A1, Electron dispersive spectroscopy confirms the presence of elemental CaPO4. A2, Semiquantitative RT-PCR. RT-PCR using the total RNA for the bone markers demonstrating an increase in the RNA expression in all markers except alkaline phosphatase. B1, Immunogold electron microscopy. The arrow points to the alkaline phosphatase gold label in the calcified aortic valves, demonstrating a marked increase in label. B2, Electron microscopy. The arrow points to an area of mineralization in the valve (34K).

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