FIG. 1.

Time course of TBI-induced expression of TIMP-3 mRNA. RT-PCR was used to measure relative levels of TIMP-3 mRNA in the ipsilateral cortex (A) and contralateral cortex (B), and the ipsilateral (C) and contralateral (D) hippocampus of the rats in the three study groups. The relative expression levels for each gene of interest were calculated by normalizing the quantified cDNA transcript level (cycle threshold, Ct) to that of GAPDH (Sham, sham-injured; TBI + No, TBI and normothermia; TBI + Hy, TBI and hypothermia). Data represent mean ± SD (n = 5; ^#p < 0.01 hypothermia and normothermia groups versus sham-operated group; *p < 0.01 hypothermia group versus normothermia group).