2.
Formation of bone marrow progenitor colonies in from AI-prone MRL-lpr/lpr and control non-autoimmune mice
| Group description | Visual symptoms | Group number | Number of mice | Number of colonies* | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| BFU-E | CFU-GM | CFU-GEMM | |||||||||||||||
| CBA (3–7 months) | no | 1 | 8 | 3.0 ± 0.5** | 7.3 ± 1.0** | 0.25 ± 0.05** | |||||||||||
| MRL-lpr/lpr males | |||||||||||||||||
| Healthy (2–3 months)ζ | no | 3 | 5 | 6.5 ± 1.5 | 7.0 ± 1.0 | 0.5 ± 0.1 | |||||||||||
| Healthy, pre-diseased (7 months)ζ | no | 5 | 5 | 12.7 ± 1.4 | 30.0 ± 1.3 | 9.2 ± 1.9 | |||||||||||
| Diseased (7 months) | yes | 7 | 5 | 25.3 ± 9.8 | 7.4 ± 0.4 | 3.9 ± 2.0 | |||||||||||
| Immunized (3 months) | yes, weak | 9 | 5 | 7.0 ± 2.1 | 6.0 ± 2.6 | 0.9 ± 0.7 | |||||||||||
| MRL-lpr/lpr females | |||||||||||||||||
| Healthy (2–3 months)ζ | no | 4 | 5 | 5.5 ± 0.5 | 11 ± 2.5 | 0.5 ± 0.2 | |||||||||||
| Healthy, pre-diseased (7 months)ζ | no | 6 | 5 | 11.5 ± 2.0 | 23.0 ± 3.0 | 8.2 ± 3.0 | |||||||||||
| Diseased (7 months) | yes | 8 | 5 | 22.1 ± 8.0 | 9.0 ± 3.9 | 2.4 ± 1.8 | |||||||||||
| Pregnant (3 months) | no | 10 | 5 | 6.8 ± 2.0 | 7.8 ± 1.5 | 0.1 ± 0.08 | |||||||||||
| Lactating (3 months), 4 days after delivery | no | 11 | 5 | 8.8 ± 2.0 | 19.1 ± 1.8 | 0.25 ± 0.2 | |||||||||||
| Lactating (3 months), 14 days after delivery | no | 12 | 5 | 21.0 ± 8.0 | 9.7 ± 0.5 | 2.1 ± 0.7 | |||||||||||
ζ
MRL-lpr/lpr mice demonstrating no SLE indexes and showing these indexes similar to healthy control non-autoimmune mice were conditionally designated (independently of age) as healthy MRL-lpr/lpr mice; similar animals demonstrating detectable levels of abzymes were conditionally designated as pre-diseased. ΦCohorts with statistically significant (P≤ 0.05) differences in the parameters in comparison with conditionally healthy MRL-lpr/lpr males and females are given in boldface.