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2.

2

A: real-time fluorescence measurements by PCR using the LightCycler instrument (Roche Diagnostics) targeting the UL30 gene of HSV; GFP was used as a DNA extraction control. SNTC = sample negative template control; GFP PC = Green Fluorescent Protein positive control; GFP patient = clinical specimen spiked with GFP; HSV PC = HSV positive control; HSV patient = patient specimen results. (B) real-time fluorescence measurements by PCR using the LightCycler instrument (Roche Diagnostics) targeting the HSV-1 gD and the HSV-2 gG. HSV-1 patient = HSV-1 detected in patient specimen; HSV-2 patient: HSV-2 detected in patient specimen.