Fig 7.

Analysis of TGF-β₁ in astrocytes expressing exon-1 htt 72Q. Real Time RT-PCR of TGF-β₁ mRNA analysis (A) and FACS analysis of intracellular TGF-β₁ protein (B, C) in astrocytes transfected with exon-1 of human huntingtin (htt) containing an expanded 72Q repeat or an unexpanded 25Q repeat used as a control, under basal conditions and after treatment with LY379268, 1 μM for 10 min. The mean fluorescence intensity (MFI) of TGF-β₁ in astrocytes transfected with exon-1 htt 25Q or exon-1 htt 72Q and treated with PBS or LY379268 was unchanged (MFI: 22.4 ± 5.8, 22.1 ± 5.7, 24.5 ± 6.7, 20.6 ± 6.3, respectively). Values are means ± S.E.M. of five to six determinations. P < 0.05 versus the respective values obtained in transfected astrocytes treated with PBS (*) or versus the respective values obtained in astrocytes transfected with exon-1 htt 25Q (#) (one-way anova+ Fisher’s PLSD).