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Release of sCD40L upon co-culture of HIMEC with PLT from healthy controls and patients with active IBD. PLT from either healthy controls (n= 8; empty columns) or patients with active IBD (8 UC and 9 CD; shaded columns) were co-cultured with HIMEC that were either left untouched or activated with TNF-α. The release of sCD40L was measured with conventional ELISA in culture supernatants. The bar graph (mean ± S.D.) summarizes the results obtained in six independent experiments.
