Fig 5.

The trilineage differentiation potential of TAFs was assessed by immunofluorescence staining for markers specific for osteoblasts (A– osteocalcin, green fluorescence), chondroblasts (B– aggrecan, red fluorescence) and adipocytes (C– FABP4, red fluorescence). Nuclear counterstaining was performed in all cases with DAPI (blue fluorescence). Both TAFs and MSCs (photos not shown) followed a similar differentiation pattern towards osteoblasts and chondrocytes, whereas differentiation to the adipocytic lineage was 30% less efficient for TAFs compared to MSCs. Magnification, 200x.