Fig 4.

Hypoxia-induced rapid MIF release is not affected by cycloheximide or echinomycin. HUVECs (15,000 cells) were incubated overnight in 24-well plates in a normoxic incubator. At t= 0, media were replaced by hypoxic media either containing 5 μM glyburide, 2 μg/ml cycloheximide or 10 nM echinomycin. Solvent was used as a control. MIF concentrations were determined from 1 hr supernatants by ELISA. Bars represent mean values (n= 3) ± S.D. Asterisks, ***, indicate statistical significance with P < 0.005.