FIG. 8.

Neuron Fraction in CA3 at PID2. (A) Schematic representation of the regions of interest (ROIs: CA1, CA3, DG) drawn for export to Imaris. (B) Representative image of colored spheres resulting from spots analysis of a CA3 ROI. Caspase+ cells are shown in this schematic as blue spheres, NeuN+as red spheres; cells positive for both markers are shown as purple spheres, for computerized counting of all spheres present in the ROI. (C) Representative image of Ipsilateral CA3 at higher magnification (60X). NeuN positive cells are shown in blue, while SYTOX positive are shown in red. CA3 neuron fraction increased in EPOCCI rats, relative to VEHCC. CA3 neuron fraction in EPOCCI rats did not differ from SHAMVEH. Scale bar: 60 μm. Color image is available online at www.liebertpub.com/neu