(A–D) Normalized thermogenic gene expression in BAT (A and B) and SubQ adipose tissue (C and D) from Adipo-PRDM16 KO and control mice at RT and following 48 hours at 4°C (A and C) or following five daily injections of 1 mg/kg CL (B and D). Cold exposure was done with male 6–8 week old mice, n=5–6 per group. CL treatment was done with female 6–8 week old mice, n=5–6 per group. Data are presented as mean ± SEM. *, p< 0.05 KO vs. control cold exposed or CL treated. #, p< 0.05 KO vs. control room temperature or untreated.
(E) Representative images from UCP1 immunohistochemistry on sections of interscapular BAT or inguinal SubQ adipose tissue from Adipo-PRDM16 KO and control mice at RT or following 48 hours at 4°C. Images are shown at 10x magnification. Scale bar =100 microns.
See also Figure S2.