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. 1984 Dec;81(24):7897–7901. doi: 10.1073/pnas.81.24.7897

Immunization in vitro and production of monoclonal antibodies specific to insoluble and weakly immunogenic proteins.

J Van Ness, U K Laemmli, D E Pettijohn
PMCID: PMC392260  PMID: 6083563

Abstract

A procedure is described for immunizing in vitro and stimulating proliferation of specific B-cell lymphocytes. The method is applicable to production of monoclonal antibodies against proteins that are soluble only in denaturing solvents. An induction period is described in which antigen is presented to the B-cell population in the absence of serum. Also, antigen is coupled to mitogenic silica, which allows the effective presentation of both soluble and insoluble antigens. The results indicate hybridomas can be obtained that secrete IgMs directed against highly conserved or weakly immunogenic antigens.

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Selected References

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