Table 1.
Primer pairs derived from the chloroplast genome of G. tenuistipitata var. liui
| Name | Forward primer (5′-3′) | Reverse primer (5′-3′) | Repeat motif | Predicted product size (bp) | Genomic coordinates of the SSR loci | Ta (°C) | Coding/non-coding region |
|---|---|---|---|---|---|---|---|
| GT1 |
TTTATCAACGATCCCTGTAG |
AATGGACTGTAATTCACCAA |
(T)10 |
336 |
4314 – 4323 |
52 |
Non-coding |
| GT2 |
TTTTTGAGCGATATTTTGAC |
AGAATAAGACCACCTGAACC |
(T)10 |
310 |
78590 – 78599 |
53 |
Coding |
| GT3 |
CCATAATGGAGATCTGTTTG |
CTGGCAACATAGTTAGCATT |
(T)10 |
306 |
84904 – 84913 |
53 |
Coding |
| GT4 |
AGCAATCCTAAATTGACAAC |
AAGGTAGACCAGGAGAAAAA |
(ATT)5 |
295 |
99538 – 99552 |
53 |
Coding |
| GT5 |
AGAAATTGATCAAGCTGTT |
TTTTCAGCAATTGGAGTATC |
(AT)8 |
329 |
105129 – 105144 |
52 |
Non-coding |
| GT6 |
CCTACAATCAGAATGGAATG |
AGCTTCCAAGAAAAATGAGT |
(A)10 |
303 |
143312 – 143321 |
53 |
Coding |
| GT7 |
ATCCTTCTTTTAAGCCGTAG |
TCTTCCATGAAGTCTTCTTTT |
(T)10 |
309 |
161988 – 161997 |
53 |
Non-coding |
| GT8 | CTCCTGACATGATAAACACC | CTTCGATTTGTTTAATGAGC | (AT)7 | 288 | 179473 – 179486 | 52 | Non-coding |
Ta Annealing temperature.