Figure 2.

Representative staining of day 21 agarose and RAD hydrogels seeded with bovine bone marrow stromal cells (BMSCs) and cultured with TGF or TGF + dexamethasone (Dex) medium. (A) Toluidine blue staining for proteoglycans, (B) type II collagen immunohistochemistry, (C) hematoxylin staining for nuclear blebbing (arrows indicate apoptotic cells), and (D) percentage of apoptotic cells, n = 3; values are mean ± standard error of the mean. Controls performed on bovine osteochondral explants. Scale bar is 30 µm.