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. 2013 Jan;4(1):63–74. doi: 10.1177/1947603512455196

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Figure 4. — Analysis of aggrecan cleavage products extracted from day 21 agarose and RAD hydrogels seeded with bovine bone marrow stromal cells (BMSCs) and cultured in TGF medium (T) ± RU-486 (R) and TGF + Dex (D) ± RU-486 medium. Ten micrograms sGAG loaded per lane. (A) anti-NITEGE Western blot and (B) anti-G1 Western blot. Arrows in (B) correspond to (a) full-length aggrecan, (b) potential m-calpain cleavage fragment, (c) G1-NITEGE fragment, and (d) potential link protein. Anti-NITEGE blot was stripped and reprobed with anti-G1 antibody to obtain image B. Representative of two repeats for each experiment type.