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. 2013 Jan;4(1):63–74. doi: 10.1177/1947603512455196

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Figure 5. — Extracellular matrix and cellular content in agarose and RAD hydrogels seeded with human bone marrow stromal cells (BMSCs) and cultured in TGF or TGF + Dex medium. (A) Sulfated glycosaminoglycan (sGAG) content, (B) DNA content, (C) proteoglycan synthesis, (D) hydroxyproline content, (E) percent sGAG retained, (F) Western blot of aggrecan extracted from day 21 RAD gels seeded with human BMSCS and cultured in TGF (T) and TGF + Dex (D) medium. Top blot is anti-NITEGE, lower blot is anti-G1. Twenty micrograms sGAG loaded per lane. Values are mean ± standard error of the mean. n = 11-17 (3-6 hydrogels × 2-3 patients). †Versus TGF, §versus day 14, P < 0.05.