Table 2.
Output format for forward primers
|
ssc
-let-7a |
tgaggtagtaggttgtatagtt |
|
|
|
|
|
|
|
|
|---|---|---|---|---|---|---|---|---|---|
|
Primers |
|
|
|
|
|
|
|
|
|
| Name | Seq | Length | score | two_last_score | three_last_score | five_last_score | three_self_anneal | internal_self_anneal | five_prime_score |
|
F_1 |
gcagtgaggtagtaggttgt |
16 |
0.64 |
1.0 |
1.0 |
1.0 |
1.0 |
1.0 |
10 |
|
F_2 |
gcagtgaggtagtaggttg |
15 |
0.56 |
1.0 |
1.0 |
1.0 |
1.0 |
1.0 |
10 |
|
F_3 |
cgcagtgaggtagtaggt |
13 |
0.42 |
1.0 |
1.0 |
1.0 |
1.0 |
1.0 |
10 |
|
F_4 |
cgcagtgaggtagtaggtt |
14 |
0.34 |
0.7 |
1.0 |
1.0 |
1.0 |
1.0 |
10 |
|
F_5 |
gcagtgaggtagtaggttgta |
17 |
0.25 |
0.7 |
1.0 |
0.5 |
1.0 |
1.0 |
10 |
|
F_6 |
cgcagtgaggtagtagg |
12 |
0.25 |
0.7 |
1.0 |
1.0 |
1.0 |
1.0 |
10 |
|
F_7 |
gcagtgaggtagtaggttgtat |
18 |
0.09 |
0.7 |
0.3 |
0.5 |
1.0 |
1.0 |
10 |
| F_8 | gcagtgaggtagtaggttgtata | 19 | 0.02 | 0.7 | 0.3 | 0.5 | 0.2 | 1.0 | 10 |