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. 2010 Apr 7;15(4):809–824. doi: 10.1111/j.1582-4934.2010.01069.x

Fig 6.

Fig 6

Effect of IL-1β, overexpression of p65 and knockdown of p65 on mRNA expression of selected genes. Myometrial cells were incubated with IL-1 (1 ng/ml) for 24 hrs after which total RNA was extracted and mRNA expression of selected genes was measured by qPCR. Note the logarithmic scale. (A) Mean ± S.E.M. n= 6, *P < 0.05 Wilcoxon matched pairs analysis. Myometrial cells were transfected with the expression construct for p65. The empty expression vector pSG5 was used as control. After 24 hrs, total RNA was extracted and mRNA expression of selected genes was measured by qPCR. Note the logarithmic scale. (B) Mean ± S.E.M. n= 7, *P < 0.05 Wilcoxon matched pairs analysis. Myometrial cells were transfected with either siRNA for p65 or a non-targeting siRNA control. After 72 hrs cells were incubated with IL-1 (1 ng/ml) for a further 24 hrs, after which total RNA was extracted and mRNA expression of selected genes was measured by qPCR. Note the linear scale. (C) Mean ± S.E.M. n= 6, *P < 0.05 Wilcoxon matched pairs analysis.