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IL-1β effect on MLCK promoter activity. IL-1β effect on the activity of the full-length MLCK promoter region (2091bp) in Caco-2 monolayers. pGL-3 basic vector containing the MLCK promoter region was transfected into the filter-grown Caco-2 cells. Caco-2 cells were treated with IL-1β (10 ng/ml) for 6 hrs. The MLCK promoter activity was determined by the luciferase assay and expressed as relative luciferase activity (means ± S.E., n= 8). *P < 0.0001 versus control.
