Fig 6.

ELISA-based DNA binding assay of transcription factors Elk-1, AP-1 and STAT-1. (A and B) IL-1β treatment caused a significant increase in Caco-2 Elk-1 and AP-1 binding to the DNA probe. (C) IL-1β did not affect STAT-1 binding to its DNA binding site in Caco-2 monolayers (means ± S.E., n= 4). *P < 0.0001 versus control. Relative activity of transcription factors was determined by comparing the relative binding of the activated transcription factor of interest to its DNA binding site following IL-1β treated cells versus control cells.