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Elk-1 knockdown by siRNA transfection prevented the IL-1β-induced increase in MLCK promoter activity as assessed by luciferase activity. Caco-2 monolayers were co-transfected with siRNA Elk-1 for 96 hrs before IL-1β treatment (means ± S.E., n= 8). *P < 0.0001 versus control; **P < 0.0001 versus IL-1β treatment.
