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. 2014 Jan 30;45(1):10. doi: 10.1186/1297-9716-45-10

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Copyright © 2014 Regidor-Cerrillo et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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IgG humoral immune responses. Serum levels of total IgG (a), IgG1 (b) and IgG2 (c) antibodies against N. caninum. Immunoglobulin levels are expressed as a relative index percent (RIPC) according to formula: RIPC = (OD_405nm sample - OD_405nm negative control)/(OD_405nm positive control - OD_405nm negative control) × 100. Each point represents the mean + SD at the different sampling times. (*) Indicates significant differences (P < 0.05) between the two infected groups (G1 and G2). Data for 49 dpi are not represented because only one heifer from G1 and two heifers from G2 maintained pregnancy.