Table 1.

Primers and condition of amplification used in the study

Primers	Primer sequence (5′-3′)	Amplification condition	Gene	Reference
DupA1	CGTGATCAATATGGATGCTT	35 cycles: 45 s, 94°C; 45 s, 52°C and 45 s, 72°C	dupA	Gomes et al.[6]
DupA2	TCTTTCTAGCTTGAGCGA
Cag1	ATGACTAACGAAACTATTGATC	40 cycles: 1 min, 94°C; 1 min, 53°C and 1 min, 72°C	cagA	Rasmussen et al.[9]
Cag2	CAGGATTTTTGATCGCTTTATT
Hpx1a	CTGGAGARACTAAGYCCTCC	40 cycles: 1 min, 94°C; 1 min, 59°C and 1 min, 72°C	16S rRNA	Scholte et al. [12]
Hpx2	GAGGAATACTCATTGCGAAGGCGA
SA	ATGGAAATACAACAAACACAC	40 cycles: 45 s, 94°C; 45 s, 54°C and 45 s, 72°C	vacA-s	Atherton et al. [13]
SCa	CCTGARACCGTTCCTACAGC			Doorn et al. [14]
MAa	CACAGCCACTTTYAATAACGA	35 cycles at: 45 s, 94°C, 45 s, 55°C and 1 min, 72°C	vacA-m	Doorn et al. [14]
MB	CGTCAAAATAATTCCAAGGG

•Genotype vacA: s1/m1 – strain 60190 of H. pylori (GeneBank U05676).

•Genotype vacA: s2/m2 – strain Tx30a of H. pylori (GeneBank U29401).

•^aR = a A or G and Y = C or T.