Table 1.
Average distribution of AIV-nucleoprotein antigen determined by immunohistochemistry (IHC) in tissue samples
|
VIRUS STRAIN |
Time of AIV NP antigen detection |
|
Main localization |
||
|---|---|---|---|---|---|
| Tissue | 1 dpi | 2 dpi | 3 dpi | 4 dpi | |
| FPV |
|
|
|
|
|
| CNS |
- |
- |
+ |
+ |
Neurons, glial cells |
| Heart |
- |
+ |
+ |
+ |
Myocytes |
| Kidney |
- |
- |
−/+ |
+ |
Epithelialtubularcells |
| Pancreas |
- |
- |
+ |
+ |
Exocrine acinar cells |
| Liver |
- |
- |
+ |
+ |
Kupffer’s cells |
| Spleen |
- |
- |
+ |
+ |
Macrophages |
| Thymus |
- |
+ |
+ |
+ |
Macrophages |
| Bursa of Fabricius |
- |
- |
+ |
+ |
Macrophages |
| FPV NS GD |
|
|
|
|
|
| CNS |
- |
- |
++ |
+ |
Neurons, glial cells, ependymalcells |
| Heart |
- |
++ |
+ |
+ |
Myocytes, macrophages |
| Kidney |
- |
+++ |
++ |
++ |
Epithelial tubular cells |
| Pancreas |
- |
- |
++ |
+ |
Exocrine acinar cells |
| Liver |
- |
- |
+ |
+ |
Kupffer’s cells |
| Spleen |
- |
+ |
+ |
+ |
Macrophages, Endothelial cells |
| Thymus |
- |
+ |
+ |
+ |
Macrophages, Endothelial cells |
| Bursa of Fabricius |
- |
- |
+ |
+ |
Macrophages, Endothelial cells |
| FPV NS VN |
|
|
|
|
|
| CNS |
- |
- |
++ |
+ |
Neurons, glial cells, ependymalcells |
| Heart |
- |
++ |
+ |
+ |
Myocytes, macrophages |
| Kidney |
- |
- |
++ |
++ |
Epithelial tubular cells |
| Pancreas |
- |
- |
++ |
+ |
Exocrine acinar cells |
| Liver |
- |
- |
+ |
+ |
Kupffer’s cells |
| Spleen |
- |
- |
+ |
+ |
Macrophages, Endothelial cells |
| Thymus |
- |
- |
+ |
+ |
Macrophages, Endothelial cells |
| Bursa of Fabricius |
- |
- |
+ |
+ |
Macrophages, Endothelial cells |
| Saline Solution |
|
|
|
|
|
| CNS |
- |
- |
- |
- |
- |
| Heart |
- |
- |
- |
- |
- |
| Kidney |
- |
- |
- |
- |
- |
| Pancreas |
- |
- |
- |
- |
- |
| Liver |
- |
- |
- |
- |
- |
| Spleen |
- |
- |
- |
- |
- |
| Thymus |
- |
- |
- |
- |
- |
| Bursa of Fabricius | - | - | - | - | - |
Chickens were inoculated with FPV, FPV NS GD or FPV NS VN and necropsies were performed at different time-points. The extension of the staining in tissues was measured by a semi-quantitative score: no positive cells (−), single positive cells (+), scattered groups of positive cells (++), and widespread positivity (+++).