Table 1.
List of sequences and primers designed to perform SemiQ RT-PCR experiments
|
GST class |
Sequence ID |
Primer features |
|||
|---|---|---|---|---|---|
| Forward (5′-3′) | Reverse (5′-3′) | Annealing temperature (°C) | Expected amplicon (nt) | ||
| Tau |
CITSI24:CX077363 |
TCGGCATGGAAGAAGTGAAGCTACTGGGA |
ACCGTTCAAAAGATAGTGTCTATGAAGTA |
65 |
784 |
| CITSI47:3 |
TCGCCAAGCCCATTTGTGATGAGGGCA |
ACGAGACAGGCTGCTGCCTAGTCCGA |
66 |
866 |
|
| CITSI57:1 |
AGTAAGCTTCTGTAATAATGGCGGACGA |
ACAATACCCTAAGATAACAGTCGGGGACA |
64 |
879 |
|
| CITSI43:1 |
TCTGTCACAATGGCGGACGAAGTGGT |
AGCAGGCAGCACGATTGCGCTGCT |
68 |
732 |
|
| CITSI16:1 |
TCACTCGCCCTTAATTCTCAGTAAGGT |
AGATTGACGCCACATAATATTCCCA |
60 |
966 |
|
| CITSI53:1 |
TGCTGGGTTACTGGGCAAGCCCCT |
ACCTTCATGCATGGGCAACCGCTGA |
66 |
686 |
|
| CITSI41:1 |
GTTTACAGGGTGATTTGGGCTCTGA |
ACCACTATGCTAGTCCCCCGAACT |
63 |
757 |
|
| CITSI25:1 |
AAGCTCTATGGCACATGGGTAAGCCCTT |
ACACAGAGAGAGAGCTAACCCAATCA |
65 |
730 |
|
| CITSI51:1 |
TGGCCAAGCCCGTTTGTGTTTAGGGT |
AGACTTTCCACACAACATCACACTAC |
63 |
756 |
|
| CITSI36:1 |
GCGAAAATAATATGGCCAAAGAAGTGACGCT |
GTCATTACAACACACCACAACACCACCT |
64 |
765 |
|
| CITSI04:1 |
AGACGTGGTCAAGCCCCTTTGGT |
TGGAATGGGAAAAGGGCAAAAGGA |
62 |
889 |
|
| CITSI44:1 |
GCAGAAGATTATGGCAACAAAAGTG |
GAGCGTACAGAAAGGAGACACGTGCA |
62 |
764 |
|
| Phi |
CITSI52:1 |
AAGCTCTTCGAATCAAGGGCAATCACCCAA |
TTTTGATAAACCCATTGGGACAGTCGT |
65 |
800 |
| CITSI38:CK934228 |
ATACGAATCTCGAGCTATCATAAGGTACTA (a) |
TTTTCTCCCAAGGCCCCAAGCATTT |
64 |
636 |
|
| CITSI23:1 |
ATACGAATCTCGAGCTATCATAAGGTACTA (a) |
TTTTGTCCTCCAAGGCCCCAAGCAT |
64 |
638 |
|
| CITSI34:CK939385 |
ATACGAATCTCGAGCTATCATAAGGTACTA (a) |
TTTTGTCCTCCAAGGCCCCCAGCAT |
65 |
678 |
|
| CITSI33:1 |
TTTATACGAGTCGCGAGCTATCATGAGGT |
TTTTAAAGCTCCAACTCCAAACAT |
60 |
658 |
|
| CITSI05:DY257328 |
ATTTTATACGAGTCGCGAGCTATCATGAGG |
ACCCCTTATCCAAGGAACATTTCCCA |
64 |
565 |
|
| CITSI11:1 |
TGGGGATTTTACTCTATACGAATCGCGA |
ATGGCGACAACAAGAAATCGCCGCA |
63 |
640 |
|
|
DQ198153 (CITSI02:1 + CITSI00:1) |
GCTTTTTGAGTCGAGGGCAATCATAAGG |
GATAACAGTAATGACAGCCAGCCGAA |
64 |
549 |
|
| CITSI29:1 |
AGGATGAGAAAATCTCCCTCTTAGAGTCT |
TGGGAAATTATTAGACCATGCCA |
63 |
748 |
|
| CITSI18:1 |
ATTTGAATCTAGAGCAATGACAGCATACGT |
CATCAATGTAAAATCATCACGCAACCA |
63 |
599 |
|
| Lambda |
CITSI46:1 |
CCTCCAAGATAGGCCCGCTTGGTAC |
TCCAGCAACGTACACAAGCTCACATCGGCA |
66 |
672 |
| Zeta |
CITSI21:1 |
ATGCTGAAACTGTATTCATACTGGAGGAGT |
TGCTGCTTATTGAGGGTCAACAAAGGCTG |
64 |
880 |
| Mapeg | CITSI20:1 | CGACTCGACTATGGCGGATGCAAC | CTATGAGCTTATGCTTGCGCCATGCAGC | 66 | 638 |
GST classes, oligonucleotides sequences and the expected size of amplicons (on the basis of correct maturation) are highlighted. Uppercase letters indicated the use of the same forward primer.