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. 2013 Aug 28;16(5):657–672. doi: 10.1111/cmi.12181

Figure 1.

Figure 1

Identification of RON 12, a novel Plasmodium-specific protein expressed in blood-stage parasites.A. clustalw alignment of RON12 sequences from P. falciparum (Pf), P. yoelii (Py), P. berghei (Pb), P. knowlesi (Pk) and P. vivax (Pv). Identical residues are highlighted in black, residues conserved in three or more species are highlighted in grey. Green box indicates putative signal peptides; conserved cysteines are boxed in red.B. Immunoblot of purified schizont (S), merozoite (M), uninfected erythrocyte (RBC) extracts and culture supernatant (CS) probed with RON12-specific antibodies.C. Analysis of protein solubility: schizonts and merozoites were serially extracted in hypotonic, high-salt or -carbonate buffer [resulting in membrane-associated (carb sol) or integral membrane protein fraction (carb pellet)] followed by SDS-PAGE and immunoblotting with the antibodies indicated. Total parasite lysate was used as immunoblotting control. Sizes of molecular mass markers (kDa) are shown on the left.