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. 2014 Feb 12;9(2):e88571. doi: 10.1371/journal.pone.0088571

Figure 7. Notch activation by DLL1 in POFUT1 deficient fibroblasts.

Figure 7

Western Blot analysis of cell lysates of NOTCH1 expressing HeLa cells (HeLaN1) co-cultured with POFUT1+/+ or POFUT1−/− fibroblasts or with CHO cells over-expressing DLL1 using the anti-Cleaved Notch1 (Val1744) antibody, which specifically detects the Notch1 intracellular domain (NICD) after S3 cleavage. Non-fucosylated DLL1 can efficiently activate Notch1.