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. 2014 Feb 12;9(2):e88642. doi: 10.1371/journal.pone.0088642

Figure 2. Kv1.3 channel involved in Meth mediated microglial damage.

Figure 2

The cells were pre-incubated with 4-AP (1 mM), TEA (5 mM) and MgTx (10 nM) for 30 min before addition of Meth (300 µM). After the treatment of Meth for 24 h, the cell viability was evaluated by the CCK-8 assay. The toxic effect of Meth was dose-dependent, with a significant reduction at 300 and 1000 µM. The injury was partly retarded by the Kv antagonist 4-AP, TEA and MgTx. LPS (1000 ng/ml) was used for the positive control. Absorbance was measured at a wavelength of 450 nm using a microplate reader. Each data point represents mean ± S.E. of at least three separate experiments in which treatments were performed in quadruplicates. * indicates significant difference when the values were compared to that of the control. (*p<0.05) and # indicates significant difference when the values were compared to that in the Meth treated group (300 µM).