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. 2014 Feb 12;9(2):e88633. doi: 10.1371/journal.pone.0088633

Figure 3. rad52▵ tlc1▵ ctf18▵ haploid strain requires RAD52 for viability.

Figure 3

[A]: The CP1 diploid heterozygous for tcl1::LEU2, ctf18::KanMX, and rad52::TRP1 were transformed with the RAD52-containing CEN plasmid, pWJ1213, giving rise to CP2. CP2 cells were grown on solid YPD medium for 72 hours to allow for plasmid loss. Single colonies were then patched onto both histidine-omission media and YPD to determine the rate of plasmid loss. [B]: Products of random spore analysis of CP2 grown on YPD for 72 hours in an attempt to isolate a viable haploid tcl1::LEU2, ctf18::KanMX, and rad52::TRP1 strain. Of the 75 triple mutant haploid spores tested, none were able to lose the plasmid, as evidenced by continued growth on histidine-omission media, and retention of growth on YPD.