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. 2014 Feb 12;9(2):e88747. doi: 10.1371/journal.pone.0088747

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© 2014 Toyonaga et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Interaction of gpMincle with gpFcRγ. HEK293T cells were transfected with HA-tagged gpMincle alone or together with Flag-tagged gpFcRγ Total lysates were immunoprecipitated with anti-HA mAb and blotted with anti-Flag and anti-HA polyclonal antibodies (pAbs). Total lysates were also blotted with anti-Flag pAb. (B) Surface expression of gpMincle. HEK293T cells were transfected with gpMincle-HA alone or together with Flag-gpFcRγ. Surface expression of gpMincle was detected by anti-HA pAb. Data are presented as representative of two separate experiments.