Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Feb 12;9(2):e88747. doi: 10.1371/journal.pone.0088747

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Toyonaga et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(A) Immunoblot of gpMCL. HEK293T cells were transfected with HA-tagged mMCL or gpMCL. Total lysates were blotted with anti-HA mAb (left panel) or immunoprecipitated with anti-HA pAb and blotted with anti-HA mAb (right panel). (B) gpMCL is associated with gpFcRγ. HEK293T cells were transfected with HA-tagged gpMCL alone or together with Flag-tagged gpFcRγ Total lysates were immunoprecipitated with anti-HA pAb and blotted with anti-Flag pAb and anti-HA mAb. Total lysates were also blotted with anti-Flag pAb. (C) Surface expression of gpMCL. HEK293T cells were transfected with HA-tagged gpMCL alone or together with gpFcRγ-IRES-GFP. HEK293T cells were also transfected with HA-tagged mMCL together with mFcRγ-IRES-GFP. Surface expression of gpMCL or mMCL was detected by anti-HA pAb. (D) gpMCL fails to recognize TDM. Indicated reporter cells were stimulated with plate-coated TDM for 24 h. Induction of NFAT-GFP was analyzed by flow cytometry. Data are presented as mean ± s.d. (D) and representative of two or three separate experiments.