Skip to main content
. 2014 Feb 12;9(2):e88944. doi: 10.1371/journal.pone.0088944

Figure 4. Effect of leptin pre-treatment on (a) tumor necrosis factor (TNF)-α production and (b) intracellular cAMP levels in CD14+ monocytes.

Figure 4

Recombinant human (rh) leptin was added to CD14+ monocytes from non-obese adult male donors 1 h before stimulating with R-848 (5 µM) (n = 6 independent experiments). Cell-free culture supernatants were collected 6 h later for TNF-α ELISAs, and cell lysates were collected for cAMP ELISAs. Bars and error bars are median values and S.D., respectively. * p = 0.03 compared to no leptin pre-treatment. † p = 0.046 compared to no leptin pre-treatment.