Figure 4. Selective increase of Vγ4⁺ and Vγ6/Vδ1⁺ γδ T cells in lung and spleen of serpinb1a^−/− mice.

Lung (left) and spleen (right) cells of WT and serpinb1a^−/− mice were stained with GL-3, followed by antibodies for Vγ1, Vγ4, Vγ5, and 17D1, which detect Vγ5/Vδ1 and Vγ6/Vδ1. Shown are absolute counts of total, Vγ1⁺, Vγ4⁺, and Vγ6/Vδ1⁺ γδ T cells. No Vγ5⁺ cells were detected (data not shown), and thus, 17D1 antibody staining identified Vγ6/Vδ1⁺ cells. The data are means ± sem for eight mice/group in three experiments. *P < 0.05; **P < 0.01; ***P < 0.001. Similar results were obtained for Vγ1⁺, Vγ4⁺, and Vγ6/Vδ1⁺ spleen cells of 4-week-old mice (data not shown).