Figure 6. Selectively increased proliferation of serpinb1a^−/− Vγ4⁺ and Vγ6/Vδ1⁺ γδ T cells.

Freshly isolated lung (A and B) and spleen (A–D) cells of naive WT and serpinb1a^−/− mice were surface-stained with γδ subset antibodies, as in Fig. 4, and intracellularly for (A, B, and D) the proliferation marker Ki-67 and (C and D) RORγt. (A) Dot plots showing Ki-67 staining of Vγ1⁺, Vγ4⁺, and Vγ6/Vδ1⁺ subsets. (B) Ki-67⁺ cells quantified as percentage within each subset. (C) RORγt staining of Vγ1⁺ and Vγ4⁺ cells. (Left) Flow cytometry plots. (Right) RORγ⁺ cells quantified within the Vγ4⁺ subset. (D) Vγ4 cells costained for Ki-67 and RORγt. (Left) Contour plots. (Right) Quantitation of Ki-67⁺ RORγ^neg and Ki-67⁺ RORγ⁺ cells within the Vγ4 subsets. Means ± sem or representative data for four mice/group. **P < 0.01; ***P < 0.001.