Fig. 2.

Continuous measurement of Rubisco activity demonstrating the conversion of Rubisco from the inactive ER to the active ECM form by RCA. The data show the time course of the decrease in A₃₄₀ in assays linking RuBP-dependent 3-PGA formation to NADH oxidation (see Fig. 1a). Reactions contained either 0.1 mg mL⁻¹ tobacco Rubisco in the fully carbamylated ECM form plus 0.1 mg mL⁻¹ tobacco RCA (open squares), 0.1 mg mL⁻¹ tobacco Rubisco in the ER form (open triangles) or 0.1 mg mL⁻¹ tobacco Rubisco in the ER form plus 0.1 mg mL⁻¹ tobacco RCA (closed circles). All reactions were conducted at 30 °C and contained 5 mM ATP