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[1H,1H]-NOESY spectrum of d3′EBS1 recorded at 293 K in D2O; a partially deuterated sample was used. Shown is the sequential walk region containing NOE cross peaks between the base protons H6 or H8 and H1′ sugar protons, which are traced by black lines. Peaks are labeled with the residue number close to their intraresidue cross peak. (Gray arrows) Internucleotide cross peaks of very low intensity between resonances of the loop protons.
