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. 2014 Mar;20(3):321–330. doi: 10.1261/rna.042432.113

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© 2014 Calidas et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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FIGURE 3. — Primer extension analysis of RNPs containing S12 variants. Sequencing gel of 16S rRNA in the following reactions: untreated 16S rRNA (lane 1); dimethyl sulfate treated 16S rRNA (lanes 2,10); dimethyl sulfate-treated minimal complex (S4, S7, S8, S15, S16, S17, S20, 16S rRNA) (lanes 3,9); dimethyl sulfate-treated minimal complex with S12WT (lane 4), S12 full-length (lane 5), S12▵10N (lane 6), S12▵15N (lane 7), and S12▵27N (lane 8). A and G are sequencing lanes. Bars indicate changes in methylation patterns compared with complexes lacking S12 (lanes 3,9). The primers utilized in A, B, and C are 232, 683, and 1046, respectively.