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. 2014 Mar;20(3):321–330. doi: 10.1261/rna.042432.113

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© 2014 Calidas et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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FIGURE 5. — In vivo analysis of ribosomes formed in strains bearing truncations of S12. (A) Representative sucrose gradient sedimentation profiles of ribosomal subunits from ▵rpsl strains bearing pS12WT or pS12ΔXN. The 10%–40% gradients were pumped from the top to the bottom using a Biocomp Piston Gradient Fractionator and elution of the small and large subunits followed by 70S ribosomes was detected by monitoring at OD₂₅₄. Area under the curve was calculated from the cross-hatched (free SSU) and gray-shaded regions (70S) in each profile. (B) Comparison between strains of percent area occupied by free subunits (cross-hatched) and subunits within ribosomes (gray) is represented by a bar graph. (C) Agarose gel analysis of RNA content of free SSUs and ribosomes.