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. 2014 Mar;20(3):331–347. doi: 10.1261/rna.042747.113

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© 2014 Strulson et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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FIGURE 1. — Secondary structure and first derivative melt curves of WT and MT tRNA^Phe. (A) Secondary structure of wild-type (WT) tRNA^Phe. WT tRNA^Phe was prepared by in vitro T7 transcription. (B) Secondary structure of mutationally weakened tertiary structure tRNA^Phe, referred to as MT tRNA^Phe. (Red) Nucleotides mutated to remove tertiary contacts. (C) First derivative melt curves parameteric in Mg²⁺concentration for WT tRNA^Phe (closed symbols) and MT tRNA^Phe (open symbols).