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. 2014 Mar;20(3):331–347. doi: 10.1261/rna.042747.113

FIGURE 2.

FIGURE 2.

Melts of WT tRNAPhe in additives with increasing Mg2+ concentration. All melts were performed in the background of 10 mM sodium cacodylate (pH 7.0) and 140 mM KCl. (A–C, both columns) The effects of various crowding agents (20% [w/v]) in 0, 0.5, and 2 mM Mg2+, respectively. No additive (black), PEG4000 (blue), PEG8000 (red), Dextran10 (green), Dextran70 (purple), and Ficoll70 (cyan). (D–F, both columns) The effects of low-molecular-weight cosolutes (2 m, except PEG200, which was 20% [w/v]) in 0, 0.5, and 2 mM Mg2+, respectively. No additive (black), methanol (pink), PEG200 (cobalt blue), proline (orange), TMAO (light purple), and betaine (dark green). In certain panels, data were adjusted to have the same Tm as the buffer, designated as “TBuffer,” so that the effects on cooperativity are clear.