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. 2014 Mar;20(3):331–347. doi: 10.1261/rna.042747.113

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© 2014 Strulson et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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FIGURE 4. — Difference plots of melts of WT tRNA^Phe in additives minus in buffer alone with increasing Mg²⁺ concentration. All melts were performed in the background of 10 mM sodium cacodylate (pH 7.0) and 140 mM KCl. All data were adjusted to have the same T_m, designated as “T_Buffer.” (A–C) The effects of various crowding agents (20% [w/v]) in 0, 0.5, and 2 mM Mg²⁺, respectively. PEG4000 (blue), PEG8000 (red), Dextran10 (green), Dextran70 (purple), and Ficoll70 (cyan). (D–F) The effects of low-molecular-weight cosolutes (2 m, except PEG200, which was 20% [w/v]) also in 0, 0.5, and 2 mM Mg²⁺, respectively. Methanol (pink), PEG200 (cobalt blue), proline (orange), TMAO (light purple), and betaine (dark green). Difference plots show the effects of the additives on WT folding cooperativity.