Table 7.

von Frey Test 3 examined the relationship between the response and the force of the von Frey filament, across a range of filaments.

	PRO:OEST		PRO:MET		PRO:DI		OEST:MET		DI: OEST		DI:MET
	%	t =	%	t =	%	t =	%	t =	%	t =	%	t =
Pre-Surgery	−0.83	−0.88	−1.43	−1.5	−0.95	−0.83	0.59	0.69	0.20	0.98	−0.4	1.6
N0S0	0.10	−0.68	−0.10	−1.2	0.20	−0.68	−0.20	0.45	1.1	−0.67	0.9	1.2
N0S4	−1.1	−1.1	−0.60	−0.63	−1.0	−0.34	0.50	0.60	−0.80	−0.94	−0.30	−0.40
N1S3	1.7	2.2	2.1	2.4	0.20	−0.020	0.40	0.49	2.0	2.6	1.9	2.4
N3S1	2.1	3.5	2.9	3.4	0.097	0.12	−0.80	−1.4	2.1	3.3	3.0	3.0
N4S0	1.0	1.3	1.0	0.39	1.0	0.74	0.010	−0.94	0.013	0.60	−0.012	−0.40

Prior to nerve injury (Pre-Surgery), the rodent oestrus cycle did not influence female rodent responses. Following nerve injury, once allodynia became stable (days 17–21) von Frey Test 3 revealed an oestrus cycle effect, with N1S3 and N3S1 females significantly more allodynic throughout pro-oestrus and dioestrus. Results generated by von Frey Test 3 are expressed as the percent difference in response per increase in von Frey hair stiffness, generated from the estimated increase in percent response per increase in von Frey stiffness; Table A4. A t-value of < −2 or >2 was determined statistically significant (p < 0.05) when differences in percentage response were greater than 1.6%. n = 8 per oestrus phase. N, number of sciatic sutures; S, number of subcutaneous sutures; g, grams force. Di, dioestrus; Met, metoestrus; Oest, oestrus; Pro, pro-oestrus. %, the average difference in percent response; t =, is there a significant effect of oestrus cycle?