Table 1.
Cultivation conditions for Oenothera in climate controlled glasshouses or foil greenhouses without heating/cooling.
| Working step | Controlled environment | Foil greenhouse |
|---|---|---|
| Sowing | ||
| Container (internal dimension) | Seed flat (50 cm × 32 cm, depth 6 cm) | Storage container with perforated bottom (35.5 cm × 25.5 cm, depth 10.5 cm) |
| Substrate | Stender MPG-mixture for Arabidopsis thaliana1 | Stender MPG-mixture for Arabidopsis thaliana1 |
| Fertilizer | None | None |
| Plants per container | 50–100 seeds | 50–100 seeds |
| Transferring | ||
| Time (das)2 | 14–21 | 14–21 |
| Container (dimension) | Round 6 cm pot | Round 10 cm pot |
| Substrate | Stender MPG-mixture for Arabidopsis thaliana1 | Oenothera substrate3 |
| Fertilizer | 1 g Osmocote start/l substrate | 3 g/l Osmocote Exact Standard 3–4 M |
| Plants per container | 1 | 1 |
| Planting | ||
| Time (das)2 | 40–50 | 40–50 |
| Container (dimension) | Round 18-cm pots (diameter 17.5 cm, height 16.5 cm) | Round 18-cm pots (diameter 17.5 cm, height 16.5 cm) |
| Substrate | Oenothera substrate3 | Oenothera substrate3 |
| Fertilizer | 3 g/l Osmocote Exact Standard 3–4 M | 3 g/l Osmocote Exact Standard 3–4 M |
| Plants per container | 1 | 1 |
1
Composition of Stender MPG-mixture: fibric peat, vermiculite, and sand [7:2:1], supplied with 150 mg/l microelements, 100 mg/l Fe-chelate.
2
Days after sowing.
3
Composition of Oenothera substrate: peat-based substrate “Standard Potting Soil Classic” (natural clay, fibric and sod peat, supplemented with 250–450 mg/l nitrogen, 250–450 mg/l P2O2, as well as K2O 360–500 mg/l at pH 6.0), quartz sand, and fine-grained vermiculite [4:2:1]). Before sowing or transferring, the substrate is soaked with tap water containing 0.906 mg/l Propamocarb to prevent damping off.