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BAP1 phosphorylated at S592 is not associated with chromatin. (A) pS592-BAP1 is predominately nuclear. Separation of HeLa cells into S1 (cytosolic) and P1 (nuclear) fractions following 1 mJ/cm2 of UV radiation and recovery for the indicated times. (B) Nuclear pS592-BAP1 is predominately nucleoplasmic. (C) Sequential extraction of a HeLa chromatin pellet with increasing concentrations of NaCl.
