Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jan 23;2(2):145–152. doi: 10.1016/j.stemcr.2013.12.008

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

PMC Copyright notice

Derepression of Nonhematopoietic Genes in Tif1b-Deficient LSK Cells

(A) Heatmap of the expression of genes that were upregulated (541 probe sets, upper panel) or downregulated (486 probe sets, lower panel) more than 2-fold in Tif1b-deficient BM LSK cells in the microarray analysis.

(B) GSEA of the gene set specific to CD34⁻LSK HSCs in Tif1b^Δ/Δ LSK cells. Using the microarray data from CD34⁻LSK HSCs, CD34⁺LSK MPPs, Lin⁻c-KIT⁺ committed progenitors, and Lin⁺ differentiated cells, HSC-specific gene sets were selected and applied to GSEA.

(C) Upregulated genes in (A) were divided into two groups according to their expression levels in Tif1b^Δ/Δ LSK cells compared to that in control LSK cells. One group consist of probe sets that were expressed in the control LSK cells and their expression levels were enhanced upon deletion of Tif1b (“enhanced” genes). The other group includes probe sets that were not expressed in the control LSK cells but became activated in Tif1b-deficient LSK cells (“activated” genes). Bar graph shows proportions of these groups.

(D) Schematic representation of expression profiles of “Activated” genes in BM Tif1b-deficient LSK cells in (A) in various hematopoietic cell fractions, including CD34⁻LSK HSCs, CD34⁺LSK MPPs, Lin⁻c-KIT⁺ committed progenitors, and Lin⁺ differentiated cells. Expressed genes are depicted as a red bar.

(E) Proportions of probe sets in (D) that were not expressed in any of the hematopoietic cell fractions (Not expressed) and those expressed in at least one of the hematopoietic cell fractions including CD34⁻LSK HSCs, CD34⁺LSK MPPs, Lin⁻c-KIT⁺ committed progenitors and Lin⁺ differentiated cells (Expressed).

(F) Heatmap of the expression of miRNAs that were upregulated (15 miRs) or downregulated (80 miRs) more than 2-fold in Tif1b-deficient BM LSK cells in the microarray analysis.