Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jan 23;2(2):189–204. doi: 10.1016/j.stemcr.2013.12.009

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

PMC Copyright notice

PRDM1 Is Expressed in Germline Lineage Spontaneously Differentiated from hESCs

(A) qRT-PCR for the indicated mRNA isolated from H9 hESCs plated for spontaneous differentiation on the indicated days.

(B) Immunofluorescence staining of H9 hESCs plated for spontaneous differentiation with indicated antibodies on indicated days. DAPI was used as the nuclear counterstain. Scale bar, 30 μm.

(C) qRT-PCR for the indicated mRNA isolated from NTU1 hESCs plated for differentiation by adherent culture on the indicated days.

(D) Immunofluorescence staining of NTU1 hESCs with the indicated antibodies and DAPI at 5, 10, and 20 days after differentiation by adherent culture. Scale bar, 30 μm.

(E) qRT-PCR for the levels of PRDM1 and VASA mRNA in sorted EGFP⁺ cells produced by H9:OCT4-EGFP plated for differentiation on the indicated days. The experimental flowchart is illustrated. Results in (A), (C), and (E) are the mean ± SEM of three independent experiments.

See also Figure S1.