Androgens Regulate Group C Gene Expression during Terminal Differentiation
(A) Expression of group A–D genes in prostate luminal cells relative to basal cells.
(B) Changes in expression of group C genes after androgen stimulation in three published microarrays.
(C) qRT-PCR analysis of TMPRSS2 expression after 48 hr treatment with at-RA (RA) and R1881 (n = 6 for PPECs [3 BPH and 3 PCa, biological replicates] and n = 3 for cell lines [experimental replicates]).
(D) qRT-PCR for the expression of LCN2, CEACAM6, S100P, TMPRSS2, and PSA in LNCaP cells after 1 nM bicalutamide treatment for 48 hr. The data are plotted considering the untreated gene expression value as 1 (n = 3, experimental replicates).
Error bars represent mean ± SD. Box plots show minimum, 25%, median, 75%, and maximum. See also Figures S3 and S4.