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. 2014 Feb 5;15:98. doi: 10.1186/1471-2164-15-98

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Copyright © 2014 Calla et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Absolute abundance of unigenes across all libraries and total normalized counts on each library for the 40 Pfams with highest count values. The unigenes identified in the sequencing fell into one of 2,711 unique Pfam families. TMM normalized read counts on each protein family were calculated. Viral protein families were removed to overcome expression bias. Also, a family of peptidases (PF12381) with the sixth most abundant normalized count numbers was removed as 98% of the counts were derived from a single library, clearly representing an outlier of a single replication. Chitin binding proteins were the most abundant (PF 00379) followed by a family of olfactory receptors (PF01395).