Table 3.
Activity profile of the active glycoside hydrolase enzymes
| Gene ID | 01 | 02 | 03 | 05 | 06 | 07 | 08 | 09 | 11 | 14 | 15 | 16 | 17 | 18 | 19 | 24 | 25 | 26 | 29 | 30 | 36 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
T
opt
(°C) |
45 |
90 |
75 |
70 |
65 |
70 |
90 |
90 |
60 |
70 |
70 |
80 |
60 |
95 |
80 |
55 |
95 |
95 |
65 |
50 |
95 |
|
Temp (°C) ≥80% activity |
45–50 |
80–90 |
75 |
60–75 |
55–70 |
65–70 |
80–90 |
80–90 |
55–60 |
60–75 |
65–70 |
75–80 |
55–60 |
85–95 |
70–85 |
50–60 |
80–95 |
85–95 |
55–70 |
45–55 |
75–95 |
|
Temp (°C) ≥50% activity |
45–55 |
70–90 |
70–80 |
45–80 |
45–75 |
60–75 |
65–90 |
65–90 |
45–65 |
50–75 |
60–70 |
70–80 |
45–60 |
70–95 |
60–85 |
45–65 |
60–95 |
75–95 |
50–70 |
45–55 |
55–95 |
|
pH
opt
|
6 |
7 |
5.5 |
7 |
6 |
6 |
5 |
5 |
6 |
6 |
6.5 |
5 |
6 |
6 |
5.5 |
6.5 |
7.5 |
7.5 |
7.5 |
6 |
6 |
|
pH ≥80% activity |
6–6.5 |
5.5–8 |
4.5–6 |
6–7.5 |
5–6.5 |
6–7.5 |
4–8 |
4–8 |
5–6.5 |
5.5–7.5 |
6–7 |
5–5.5 |
5 –7 |
4.5–7 |
4.5–7.5 |
5.5–7.5 |
4–8 |
5.5–8 |
5.5–8 |
5–7 |
6–7.5 |
|
pH ≥50% activity |
5–7 |
4.5–8 |
4.5–7 |
5.5–8 |
4.5–7.5 |
5.5–8 |
4–8 |
4–8 |
4.5–7 |
5–8 |
6–7.5 |
5–6.5 |
5–7.5 |
4–8 |
4.5–8 |
5–8 |
4–8 |
4–8 |
5–8 |
4.5–8 |
5.5–8 |
|
IL% (v/v) ≥100% activity |
0 |
15 |
40 |
35 |
5 |
10 |
0 |
0 |
10 |
5 |
0 |
35 |
0 |
5 |
10 |
10 |
40 |
30 |
5 |
25 |
35 |
|
IL% (v/v) ≥80% activity |
0 |
25 |
40 |
40 |
5 |
15 |
5 |
5 |
15 |
10 |
0 |
40 |
0 |
15 |
20 |
10 |
40 |
35 |
10 |
30 |
35 |
|
IL% (v/v) ≥50% activity |
15 |
35 |
40 |
40 |
5 |
15 |
20 |
20 |
20 |
20 |
0 |
40 |
5 |
30 |
30 |
15 |
40 |
35 |
15 |
35 |
35 |
| Max activity in IL* | 0.68 (5) | 1.1 (5) | 1.2 (40) | 2.1 (15) | 1.3 (5) | 1.2 (5) | 0.87 (5) | 0.89 (5) | 1.5 (5) | 1.2 (5) | 0.45 (5) | 5 (10) | 0.5 (5) | 1.2 (5) | 1.1 (5) | 2.1 (5) | 1.9 (15) | 2.5 (15) | 1.4 (5) | 2.5 (15) | 2 (25) |
Enzyme activity was profiled at temperatures between 45 and 95°C, pH between 4 and 8, and IL concentrations between 0 and 40% (v/v) of [C2mim][OAc]. The temperature and pH that elicited the highest activity is indicated in row Topt and pHopt, respectively. Temperature and pH ranges that permitted greater than 80% and 50% activity are indicated below the optimum value. IL-tolerance is indicated as the maximum concentration of [C2mim][OAc] that permits at least 80% and 50% enzyme activity (i.e. a value of 15 in the 80% row would indicate that 15% (v/v) of [C2mim][OAc] is the maximum concentration of [C2mim][OAc] that can be used to retain at least 80% enzyme activity). Most enzymes showed a steady decline in activity with increasing IL concentrations. *Max activity in IL is reported as the highest fold change of activity in the presence of IL compared to water and the () indicates the IL concentration (v/v) in which that highest activity as achieved. Values less than 1 indicate the enzyme is less active in IL than in water while values greater than 1 indicate the enzyme has increased activity in the presence of IL.